Energy expenditure and metabolism of male global adult onset KO mice.

<p>A, VO₂ consumed, B, VCO₂ produced, and C, energy expenditure (EE), adjusted for variation in lean mass using multiple linear regression (ANCOVA). D, respiratory exchange ratio (RER). Adult onset KO mice (n = 12) control mice (n = 10). Measurements were made over a 22-hour period during the dark and light phases in 18-week old mice. Data are expressed as mean ± SE and individual data points are shown. For details of the lean-mass adjustment made in panels A,B,C, see <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1003166#s4" target="_blank">Materials and Methods</a>, *P<0.05, **P<0.01, ***P<0.001.