Energy intake, energy output, change of body energy, and ambulatory activity.

<p>Body energy changes versus cumulative energy intake over the 19 week experiment (Panel A) and the calculated 19 week average energy output rate versus average energy intake rate (Panel B). The data points indicate individual mice from the 5 diet groups: chow diet (C), high fat diet (HF), high fat diet plus Ensure® (EN), high fat diet for 7 wk followed by a switch to chow (HF-C), and high fat diet plus Ensure® for 7 wk followed by a switch to chow (EN-C). In panel B, the dotted line is the line of identity indicating average energy balance. The solid line is the best-fit regression line for the C, HF-C, and EN-C groups (slope = 0.90±0.03, intercept = 0.97±0.44 kcal/d, R2 = 0.98) and the dashed line is the best-fit regression line for the HF and EN groups (slope = 0.92±0.02, intercept = −0.21±0.28 kcal/d, R2 = 0.99). C) The average ambulatory activity of the mice measured at weeks 7 and 19. The bars with different letters indicate significant differences of ambulatory activity within each time period (P<0.05). Panel D plots the energy intake rate over the last two weeks of the study as a function of the final body weight (slope = 0.21±0.02 kcal/g/d, intercept = 4.99±0.93 kcal/d, R2 = 0.67). The regression analysis was performed using the least square method and other statistical analyses were performed as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0005370#pone-0005370-g002" target="_blank">Figure 2</a>.