Figure 5

<p><b>Female MIC^−/− mice exhibit lower metabolic activity than their synergic controls.</b> Metabolic activity of female MIC-1^−/− and control mice with groups of 9 at age between 14–16 weeks was determined by time course of (A) respiratory exchange rate (RER), (B) energy expenditure and (C) ambulatory activity. Energy expenditure (EE) was adjusted for lean mass via ANCOVA (common lean mass = 18.72 g), EE were significantly lower measured over 24 hour in MIC-1^−/− mice (<i>p = </i>0.001, <i>n = </i>9/group, <i>repeated measures ANOVA</i>). (D) MIC-1^−/− also displayed lower total EE in time courses over 24 hour, light phase and dark phase (<i>p = </i>0.001. <i>p = </i>0.005 and <i>p<</i>0.001, respectively, <i>n = </i>9/group, t-test). (E) Physical activity in dark phase were significantly lower in MIC-1^−/− mice (<i>p = </i>0.03, <i>n = </i>9, t-test). Data are normalized to body weight and plotted as means ± SE. Significance indicated as () for <i>p<</i>0.05 or () for <i>p<</i>0.01, or () for <i>p<</i>0.001.