Mfsd2a KO mice have increased energy expenditure.

<p><i>A–D,</i> Indirect calorimetric analysis of male WT (white bars) and KO (dark bars) mice (n = 4, 12–14 weeks of age) under <i>ad libitum</i> fed (left column) and fasted (right column) conditions for <i>A</i>, VO₂, <i>B,</i> VCO₂, <i>C</i>, energy expenditure (EE), and <i>D</i>, respiratory quotient (RER). § p<0.05, # p<0.001.