Two-color flow cytometric characterization of surface marker and EGFP expression of PBMCs infected with H6R28LEP.

<p>PBMCs were activated by culture with IL-2 and plate-bound anti-CD3 monoclonal antibody for 3 d, infected with H6R28LEP, and underwent fluorescence-activated cell sorting (FACS) analysis at 4 d after infection. <b>A:</b> Positivity of CD4 and EGFP in H6R28LEP-infected PBMCs. <b>B:</b> Positivity of CD8 and EGFP under the same experimental situation as for panel A. <b>C:</b> Positivity of CD19 and EGFP in unstimulated H6R28LEP-infected PBMCs. <b>D:</b> Positivity of CD34 and EGFP in unstimulated H6R28LEP-infected CBMCs. The percentage of cells in each quadrant of the FACS profile is shown in the diagram beneath each panel. Results are representative of at least three independent experiments.