Flow cytometric analysis of activation markers on CD4 and CD8 T cells.

<p>(<b>A</b>) Representative analysis of PBMCs from infected and control subjects. Lymphocytes were gated on forward and side scatter and T cells were subsequently gated on CD4 or CD8 expression. Quadrants were set using isotype controls for CD4, CD8, CD38 and HLADR. Data represent triple-stained cells with a minimum of 10,000 counts and shown as percent of cells expressing CD4/CD38/HLADR and CD8/CD38/HLADR above the isotype control. T cells were gated as CD4 or CD8 and subsequently analyzed for CD38 and HLADR. Coinfection (HCV/HIV) triggered increased HLADR and CD38/HLADR staining. (<b>B</b>) Quantitative analysis of both T cell subsets for CD38 and HLADR and double-stained for CD38/HLADR. For CD4 and CD8 T cells, subjects were controls (C), HCV, HIV_UD and HIV-suppressed coinfected (HIV/HCV). Significance determined by Student’s t-test (*<i>p</i><0.05, **<i>p</i><0.01, ***<i>p</i><0.001).