Increased CD34+ cells in PBMNCs isolated from HHT patients.

<p>Flow cytometric analysis showed that PBMNCs consisted of cells with different size and granularity (a), monocytic/lymphocytic population stained by fluorescent dye 7-AAD indicated over 99% cell viability (b). Manually gated cells for CD34 staining analysis were performed. A representative dot plot of CD34 analysis for a healthy volunteers and a HHT patient was shown in c and d, respectively. About 0.3% CD34+ cells were identified in PBMNCs from HHT patients, which was significantly higher than that in the control samples (e). *<i>p</i><0.05, compared with healthy controls.