Flow cytometric analysis of the numbers of different subsets of B cells.

<p>PBMCs were collected from HSPN patients and healthy controls. Then, they were stained with PerCP-anti-CD19, PE-anti-CD38, APC-anti-CD86, or PerCP-anti-CD19, PE-anti-CD27, and APC-anti-CD95 (Biolegend, San Diego, CA, USA), or isotype-matched control IgG (Beckton Dickinson, San Jose, CA, USA). For further analysis of different subsets of B cells, the cells were gated initially on living lymphocytes and then on CD19⁺ B cells. (A) Flow cytometric analysis results. (B) The numbers of CD19⁺ B cells. (C) The numbers of CD38⁺CD19⁺ plasma cells. (D) The numbers of CD86⁺CD19⁺ B cells. (E) The numbers of CD38⁺CD86⁺CD19⁺ B cells. (F)The numbers of CD95⁺CD19⁺ B cells. Data are expressed as the means for individual subjects included in two separate experiments. (G) Mean fluorescence intensity of CD86 on B cells. (H) Mean fluorescence intensity of CD95 on B cells. (I) Mean fluorescence intensity of CD95 on CD27⁺ B cell subsets. (J) Mean fluorescence intensity of CD95 on CD27^- B cell subsets. The horizontal lines represent the median values. Data were analyzed by Mann-Whitney U-test.