p53β and p53γ protein modulation after treatment with camptothecin or doxorubicin.

<p>(<b>A</b>) Immunoblot analysis of p53 levels in H1299 p53γ and H1299 p53β cells after treatment with 0.5 µM doxorubicin (Dox) and 0.2 µM camptothecin (CPT) (8 hrs incubation time). Bar graphs represent the mean of four different experiments. * P-value<0.05, ** P-value<0.01. (<b>B–G</b>) Immunoblot analysis of Bax, p21, Chk1, Puma, Tigar and Mdm2 levels in H1299 p53β, H1299 p53γ and H1299 vector control cells after treatment with 0.5 µM Dox and 0.2 µM CPT (8 hrs). β-actin act as loading control, and the ratio of p53, Bax, p21,Chk1, Puma, Tigar or Mdm2 to loading control compared to value of untreated vector control cells (set to 1.0) is indicated. Bar graphs represent the mean of five (Bax), four (p21, Chk1, Tigar, Mdm2) or three (Puma) different experiments. Error bars: standard error of mean. Protein levels were compared using Student's t-test. * <i>P</i>-value<0.05, ** P-value<0.01. Brackets represent significant changes in basal protein levels relative to untreated H1299 vector control cells.