H1299 colony formation and proliferation after chemotherapy.

<p>(<b>A</b>) Colony formation assay of H1299 p53β, H1299 p53γ and H1299 vector control demonstrated significantly decreased colony formation in p53β⁺ and p53γ⁺ cells after seven-days treatment with 25 nM doxorubicin (Dox). Insert shows a magnification of selected colonies from the doxorubicin treated plates. (<b>B</b>) illustrates count of treated colonies relative to colony count of untreated cells from (A). Controls within each group have been normalized to 100 colonies, and the treatment groups have been subsequently adjusted. (<b>C</b>) Each bar represents the number of colonies within each treatment group, and statistics have been calculated based on the untreated control within each cell subtype (p53β, p53γ, wt (not transduced) or vector control (ctrl, tdTomato vector control). (<b>D</b>) ³H-thymidine incorporation assay of p53β⁺ and p53γ⁺ cells exposed for 8 hrs to 0.5 µM Dox (n = 6 experiments), 0.4 µM camptothecin (CPT; n = 2), or vehicle (DMSO) (each separate experiments have six parallels each). Cytarabine (AraC) at 0.1 µM gave no significant response. Columns represent the ratio of treated to control (DMSO) ³H-thymidine uptake. * <i>P</i>-value<0.05, **<i>P</i>-value<0.01, *** <i>P</i>-value<0.001. Error bars: standard error of mean.