p53 expression is reduced by U-II at both mRNA and protein levels in HUVECs.

<p>(A) HUVECs were treated with DOX or DOX plus U-II. HUVECs were treated 24 h later with 1 µM DOX for the indicated time, and mRNA levels of p53 were evaluated by PCR. Equal RNA loadings were documented by GAPDH (lower). (B) HUVECs were treated with 1 µM DOX for the indicated time, and protein levels of p53 were evaluated by Western blotting. (C) HUVECs were treated with DOX or DOX plus U-II. HUVECs were treated 24 h later with or without 1 µM DOX for 24 h, and protein levels of p53 were evaluated by Western blotting. Densitometric data are means ± SEM from three separate experiments. *<i>P</i> <0.05 vs. control (Ctrl); ^#<i>P</i> <0.05 vs. DOX treatment.