Reducing <i>h</i>eIF2γ expression levels or overexpressing eIF5 suppress the slow growth phenotype conferred by <i>h</i>eIF2γ overexpression.

<p>A. Reciprocal variation in growth and expression level of <i>h</i>eIF2γ in 5-FOA resistant colonies (#1 and #2) following transformation of pAV1970 into GP5012. Left, growth on YPGal medium (Day 7). Right, anti-<i>h</i>eIF2γ western blot from liquid cultures of the same transformants: lanes a and b 1x and 2x loadings, respectively. B. Transformant #1 (GP5613) was transformed plasmids pRS426, pAV1280 or pAV2015 to make strains GP5744 (vector), GP5755 (<i>GCD11</i>) and GP5758 (hc <i>TIF5</i>) respectively and grown on YPGal medium (Day 4). C. western blots showing increasing loadings (0.5X, 1X, 2X) of cell extracts from GP5758 (lanes 1–3) and GP5744 (lanes 4–6) with indicated antibodies. Tsa1p is shown as a loading control.