Sustained expression of A2UCOE-eGFP in <i>in vitro</i>.

<p>(A) hflHSC transduced with A2UCOE-eGFP, PGK-eGFP and EF1α-GFP LVs were maintained in culture for 24 days and analyzed at 3, 10, 17 and 24 days post-transduction. Expression from A2UCOE-eGFP was sustained over the 24 day period of culture. A rapid decline in PGK-eGFP (58% to 22%) and EF1α-eGFP (71% to 23%) expression was seen by Day 10. Average vector copy number per cell (VCN) at Day 17 of culture is given in italic text above the histogram lines. (B) A2UCOE-eGFP LV transduced cells showing maintenance of multilineage differentiation capability in methylcellulose culture assays at 17 days post-transduction. (C) Quantification of proportion of eGFP expressing colonies following differentiation of LV transduced hflHSC in semi-solid methylcellulose culture assays. Note comparable levels of eGFP-positive colonies with all three (A2UCOE, EF1α, PGK) LV types.