<p>A. Growth of yeast strains GP6124 (<i>y</i>eIF2), GP6461 (<i>h</i>eIF2 #1), GP6462 (<i>h</i>eIF2 #2) on glucose containing YPD medium (represses <i>h</i>eIF2 expression; top) and galactose YPGal medium (bottom; <i>h</i>eIF2 expression inducing conditions). B. Western blots of cell extracts from the same strains grown in YPGal medium.</p
<p><i>A.</i> Schematic diagram of the YCplac111-YP plasmid containing N-terminally HA-tagged eIF2A u...
Protein synthesis is principally regulated at the initiation stage in which eIF4F complex plays an i...
Translation initiation factor eIF-5A (previously named eIF-4D) is a highly conserved protein that pr...
<p>A. Table comparing yeast and human eIF2 subunit proteins. B. Cartoon depiction of plasmids pAV190...
<p>Yeast strain Jo55 lacks a chromosomal eIF4E gene and only survives on galactose-containing media ...
<p>2D-gel electrophoresis followed by Western blotting for eIF2A from BY4684 ΔeIF2A yeast transforme...
<p>A. Reciprocal variation in growth and expression level of <i>h</i>eIF2γ in 5-FOA resistant coloni...
(A) MS based quantification of EF2 levels from yeast mutants (dph2Δ, eft2Δ, dph2Δ eft2Δ) in relation...
<p>A. and B.: Coomassie blue stained SDS-PAGE gel summaries of (A) single step partial-purification ...
Recessive inherited mutations in any of five subunits of the general protein synthesis factor eIF2B ...
Recessive inherited mutations in any of five subunits of the general protein synthesis factor eIF2B ...
Yeast Saccharomyces cerevisiae S288C was incubated in YPD medium consisting of glucose 90 g/L, yeast...
<p>(A) Growth of wild-type, <i>mef2</i> deletion strain and <i>mef2</i> mutants in yeast medium cont...
A gene encoding a yeast homologue of translation elongation factor 1 gamma (EF-1 gamma), TEF3, was i...
Initiation factor eIF4G is a key regulator of eukaryotic protein synthesis, recognizing proteins bou...
<p><i>A.</i> Schematic diagram of the YCplac111-YP plasmid containing N-terminally HA-tagged eIF2A u...
Protein synthesis is principally regulated at the initiation stage in which eIF4F complex plays an i...
Translation initiation factor eIF-5A (previously named eIF-4D) is a highly conserved protein that pr...
<p>A. Table comparing yeast and human eIF2 subunit proteins. B. Cartoon depiction of plasmids pAV190...
<p>Yeast strain Jo55 lacks a chromosomal eIF4E gene and only survives on galactose-containing media ...
<p>2D-gel electrophoresis followed by Western blotting for eIF2A from BY4684 ΔeIF2A yeast transforme...
<p>A. Reciprocal variation in growth and expression level of <i>h</i>eIF2γ in 5-FOA resistant coloni...
(A) MS based quantification of EF2 levels from yeast mutants (dph2Δ, eft2Δ, dph2Δ eft2Δ) in relation...
<p>A. and B.: Coomassie blue stained SDS-PAGE gel summaries of (A) single step partial-purification ...
Recessive inherited mutations in any of five subunits of the general protein synthesis factor eIF2B ...
Recessive inherited mutations in any of five subunits of the general protein synthesis factor eIF2B ...
Yeast Saccharomyces cerevisiae S288C was incubated in YPD medium consisting of glucose 90 g/L, yeast...
<p>(A) Growth of wild-type, <i>mef2</i> deletion strain and <i>mef2</i> mutants in yeast medium cont...
A gene encoding a yeast homologue of translation elongation factor 1 gamma (EF-1 gamma), TEF3, was i...
Initiation factor eIF4G is a key regulator of eukaryotic protein synthesis, recognizing proteins bou...
<p><i>A.</i> Schematic diagram of the YCplac111-YP plasmid containing N-terminally HA-tagged eIF2A u...
Protein synthesis is principally regulated at the initiation stage in which eIF4F complex plays an i...
Translation initiation factor eIF-5A (previously named eIF-4D) is a highly conserved protein that pr...