Macroscopic comparisons of alizarin red stainings.

<p>Comparison of stainings demonstrated that calcium deposition occurred only under long time cultivation of ASCs with osteogenic medium (B and C), while cells on A were kept under same conditions without osteogenic medium. Scale bar 1 cm. A: ASCs on cover glass, cultivated for 6 weeks with medium. B: ASCs on cover glass, cultivated for 6 weeks with osteogenic medium. C: ASCs on NiTi-sheet, cultivated for 6 weeks with osteogenic medium.