ASCs on NiTi, verification of osteogenic differentiation by staining of calcium depositions and osteocalcin.

<p>A: alizarin red staining, partly coated NiTi-sheet (coated part indicated by arrow, border indicated by dot line); scale bar 2 mm. B: detail of A, uncoated part; scale bar 200 µm. C: detail of A, coated part; scale bar 200 µm. D: NiTi-nanocoated structure with osteogenic ASCs; green: osteocalcin (indicated by arrows), blue: nuclei; scale bar 50 µm.