ASCs on cover glasses, 6 weeks cultivation without osteogenic medium.

<p>Alizarin red staining as well as immunofluorescence demonstrated that there did not occur osteogenesis or mineralization without osteogenic medium as the stainings were negative for the analyzed osteo markers. A: alizarin red staining; scale bar 50 µm. B: red: osteopontin, green: fibronectin, blue: nuclei; scale bar 50 µm.