<p>Mineral deposition was examined using Alizarin Red S staining. rDPC were investigated after d3 (A), d7 (B), d14 (C) and d21 (D). Clear mineralizing nodules were observed in the osteogenically induced cultures at d21 (D). Alizarin Red S quantification demonstrated elevated calcium content by nearly 10-fold at d14 and d21 compared to d3 cultures.</p
<p>(<b>A</b>) Representative diagram of quantification of Alizarin Red stain in primary human osteob...
<p>After being cultured in osteogenic medium (Os) for 25 days, mineralized nodules were stained with...
<p>HPDLCs were assigned into LIPUS-treated group, non-treated control group and the combination grou...
<p>Only small, diffusely distributed mineral deposits are detected in phase contrast images of stain...
<p>Few mineral nodules could be observed for cells without ES. A greater number of mineral nodules w...
The alizarin red S assay is considered the gold standard for quantification of osteoblast mineraliza...
<p>Comparison of stainings demonstrated that calcium deposition occurred only under long time cultiv...
Alizarin Red S staining is the standard method to indicate and quantify matrix mineralization during...
<p>Calcium deposition is depicted by black staining. The ratio of black to white pixels was (a) 1,7 ...
<p>(<b>A</b>) ALP activity performed on day 7 of oAFMSC culture at increased concentrations of R-568...
Osteoblasts grown for 3 weeks on different samples in mineralization medium were stained with Alizar...
<p>Alizarin Red S staining of empty scaffolds maintained for 8 weeks in expansion (a) or osteogenic ...
<p>MC3T3-E1 cells were cultured for 15 days with or without NUCB2<sup>1–83</sup> and the mineralized...
a. Immunocytochemical staining of osteocalcin (green) in hASCs cultured on glass discs for 21d. b. I...
<p>Alizarin red staining as well as immunofluorescence demonstrated that there did not occur osteoge...
<p>(<b>A</b>) Representative diagram of quantification of Alizarin Red stain in primary human osteob...
<p>After being cultured in osteogenic medium (Os) for 25 days, mineralized nodules were stained with...
<p>HPDLCs were assigned into LIPUS-treated group, non-treated control group and the combination grou...
<p>Only small, diffusely distributed mineral deposits are detected in phase contrast images of stain...
<p>Few mineral nodules could be observed for cells without ES. A greater number of mineral nodules w...
The alizarin red S assay is considered the gold standard for quantification of osteoblast mineraliza...
<p>Comparison of stainings demonstrated that calcium deposition occurred only under long time cultiv...
Alizarin Red S staining is the standard method to indicate and quantify matrix mineralization during...
<p>Calcium deposition is depicted by black staining. The ratio of black to white pixels was (a) 1,7 ...
<p>(<b>A</b>) ALP activity performed on day 7 of oAFMSC culture at increased concentrations of R-568...
Osteoblasts grown for 3 weeks on different samples in mineralization medium were stained with Alizar...
<p>Alizarin Red S staining of empty scaffolds maintained for 8 weeks in expansion (a) or osteogenic ...
<p>MC3T3-E1 cells were cultured for 15 days with or without NUCB2<sup>1–83</sup> and the mineralized...
a. Immunocytochemical staining of osteocalcin (green) in hASCs cultured on glass discs for 21d. b. I...
<p>Alizarin red staining as well as immunofluorescence demonstrated that there did not occur osteoge...
<p>(<b>A</b>) Representative diagram of quantification of Alizarin Red stain in primary human osteob...
<p>After being cultured in osteogenic medium (Os) for 25 days, mineralized nodules were stained with...
<p>HPDLCs were assigned into LIPUS-treated group, non-treated control group and the combination grou...