SSa inhibits low-Mg²⁺-induced SREDs activity in cultured hippocampal neurons.

<p>(A) A representative whole-cell current-clamp recording from a neuron during pBRS (with vehicle) displaying SREDs activity (a, b), after application of SSa (1 µM; c, d), and during wash out with pBRS (e). Inserted segments (a–e, lower panel) were expansions of the original traces at the arrows indicated and showed details of the inhibitory effect on SREDs activity. (B) Bar graph representing average frequencies of SREDs in the absence (pBRS with vehicle) and presence of SSa (0.1 µM to 4 µM; n = 11; paired t-test; **<i>P</i><0.01, ***<i>P</i><0.001 vs. pBRS). (C) Log concentration-response curve for SSa on the percentage inhibition of SREDs was determined by least-square linear regression analysis (n = 11; <i>IC₅₀</i> = 0.42 µM).