Effect of sst2A receptor agonist on axonal and dendritic patterning.

<p>A–A″) Representative image of sst2A receptor localization in a primary hippocampal cell after 24 h <i>in vitro</i> in culture. Receptor immunoreactivity (green in A, A″) is present in the cell body and processes. Cell morphology is revealed by actin-binding protein phalloidin (red in A′, A″). Note that sst2A receptor immunoreactivity is also present in growth cones (insets in A–A″). B–D) Representative images of neurons from control (ctrl; B), 10 nM octreotide-treated (10 nM oct.; C) and 50 nM octreotide-treated (50 nM oct.; D) cultures. Arrows depict the axonal process which appears longer when cells are treated with 50 nM octreotide. E) Quantitative analysis reveals that the axon length (right panel) is significantly increased in the 50 nM oct. group when compared to the control group. The mean cell body surface (left panel) and the mean dendritic length (middle panel) are not modified by sst2A receptor agonist treatments. Values (mean±SEM) are expressed in relation to an arbitrary unit (100%) of the control values. *p<0.05; ns, not significant. Scale bars: A–A″, B–D, 20 µm.