Expression of ABCG2, PEPT and FECH, and ALA-mediated PpIX accumulation in various cancer cells.

<p>Human lung adenocarcinoma cells (A549), human renal cancer cells (Caki-1),human colorectal cancer cells (DLD-1),human bladder cancer cells (T24) and human histiocytic lymphoma cells (U937) were used in this experiment. <i>A,</i> Western blotting analysis of whole cell lysates of various cancer cell lines. ABCG2 was detected with a polyclonal anti-ABCG2 (Cat. #4477; Cell Signaling Technology). Actin was used as a protein loading control. A representative blot of multiple experiments is shown. <i>B</i>, Relative contents of each protein were determined by densitometric scanning and were normalized using actin. <i>C</i>, Levels of accumulated ALA-mediated PpIX in various cancer cell lines. Bar graphs show flow cytometric analysis results of cellular PpIX content. Mean intensities of fluorescence (autofluorescence values) obtained from control experiments of each cell line were normalized to 100%. Results represent the mean ± SD of three independent experiments.