Analysis of VEGFR-1 and PlGF expression in human breast cancer cell lines.

<p>(A) Lysates from different breast cancer cell lines were subjected to Western blot analysis for VEGFR-1 protein expression. HUVECs were used as a positive control. β-actin is shown as a loading control. The bar graph below shows the relative protein expression levels (VEGFR-1/β-actin) among the cell lines. Data are presented as average ± s.d. from three independent experiments. *<i>P</i><0.05. (B) Breast cancer cell line-derived conditioned media were subjected to ELISA for PlGF expression. Results are shown as average ± s.d. from three independent experiments. *<i>P</i><0.05. Conditioned media from HUVECs were included as a positive control.