Germination of Δ<i>gnb-1</i> and Δ<i>gng-1</i> mutants.

<p><b>A.</b> Conidia of wild-type (74-OR23-IVA), Δ<i>gnb-1</i> (42-8-3) and Δ<i>gng-1</i> (5-5-3) strains were harvested as described in the <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0048026#s2" target="_blank">Materials and Methods</a>. An aliquot containing 8×10⁶ conidia was spread on VM solid medium and germination monitored at 30°C over time. DIC images were obtained using an Olympus IX71 microscope with a QIClick™ digital CCD camera and analyzed using Metamorph software. Bar = 5 µm. <b>B</b>. Proportion of germinated conidia of <i>N. crassa</i> wild-type, Δ<i>gnb-1</i> and Δ<i>gng-1</i> strains at various times after inoculation onto VM medium. Error bars are ±SE for a minimum of three independent experiments (n = minimum of 100 cells for all strains). Strains are the same as in A.