Localization of GNA-1-TagRFP and GNA-3-TagRFP with RIC8-GFP in germinating conidia.

<p>Conidia from a fused strain expressing GNA-1-TagRFP or GNA-3-TagRFP and RIC-8-GFP were harvested as described in the <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0048026#s2" target="_blank">Materials and Methods</a>. An aliquot containing 8×10⁶ conidia was spread on a 100 mm VM solid medium plate. Images were captured using a Leica TCS SP5 II inverted confocal microscope. Conidia were imaged immediately after inoculation on the solid medium plate. Conidia were allowed to germinate for 6 h or 4 h at 30°C before imaging. The first two panels were merged to create the third panel. All panels are 4× zoom. Scale bar = 5 µm.