Colony growth, conidiation and germination of <i>B</i>. <i>bassiana</i> wild-type, <i>ΔBbEng1</i>, <i>BbEng1</i>^<i>OE</i> and Comp strains.

A. Colony grown for 8 days at 26°C with a cycle consisting of 15 h of light and 9 h of darkness. Fungal strains were inoculated by dropping 2 μL of conidial suspensions (1 × 107 conidia / mL) on the center of 1/4 SDAY (1:4-diluted Sabouraud dextrose agar supplemented with 1% (w/v) yeast extract), PDA, CZA and CZA replacing sucrose with glucan, trehalose, glucose, mannose, erythritol, galactose or fructose at 3% (w/v). B. Growth rates calculated based on colony growth in dimeters using linear regression method and software SPSS 17.0. C. Conidiation on CZM at indicated time. D. Conidial yield per cm2 on CZA and 1/4 SDAY at indicated time. E. Conidial germination on CZA and 1/4 SDAY. F. The mean germination time (GT50). All the data were present with mean and SD from triplicated independent experiments. Error bars denote st. dev. (SD) and values with different letters indicate statistically significant differences from different treatments (P (TIF)