Overexpression of Axin1^Q162A blocks axis formation and Wnt signaling in <i>Xenopus</i> embryos.

<p>(<b>A–C</b>) Representative phenotypes of stage 40 control uninjected embryos (<b>A</b>), <i>axin1</i>-injected embryos (500 pg), (<b>B</b>) and <i>axin1(Q162A)</i>-injected embryos (500 pg) (<b>C</b>). (<b>D</b>) Representative realtime RT-PCR of <i>sia1</i>, <i>nr3.1</i> and <i>chd</i> expression in control whole embryos (un WE), control animal caps (un cap) and caps injected with <i>wnt8</i> (1 pg) and Axin constructs (500 pg). (<b>E</b>) Realtime RT-PCR of <i>nr3.1</i> expression in animal caps injected with <i>wnt8</i> and different doses of Axin constructs (100 pg, 300 pg, 500 pg; indicated below sets of bars).