Elevating Axin produces dose-sensitive inhibition of Wg signaling, while increasing APC2 levels does not.

<p>(A) Embryonic viability of indicated genotypes. (B,C) Assessing the effect of elevating Axin or APC2 levels on Wg-regulated cell fates. (B) Range of cuticle phenotypes of embryos/larvae of each genotype—since not all genotypes are lethal, phenotypes include those of hatched larvae. (C) Representative images of cuticle phenotypes used in B. Anterior to the top. 1: Wildtype. 2: 1–2 merged denticle belts (brackets). 3: 3–4 merged denticle belts. 4: Most denticle belts merged, mouth parts still present. 5: <i>wg</i> null phenotype–denticle lawn and no head (arrow). (D) Quantification of number of rows of En-expressing cells per segment. Embryos analyzed: WT-21, AxinRNAi-5, APC2^g10- 5, wg^IG22–14, Zyg Axin- 9, Mat RFP&Axin- 11, Mat Axin- 18, Mat APC2- 12. * = p<0.05 using a one-way ANOVA test. (E-J) Representative images, En expression, as quantified in D. Anterior to the left.