Phagosomes from p110α knockdown cells show marked decreases in LAMP-1 acquisition and phagolysosome fusion.

<p><b>A)</b> Confocal microscopy of control and p110α knockdown cells shows reduced phagosomal LAMP-1 acquisition. The graph to the right shows counts of phagosomes colocalized with LAMP-1 staining. At least 100 phagosomes in each of three independent experiments were counted. Open bar = Control cells, Solid bar = p110α knockdown cells. Means +/− s.e.m. are shown. **p<0.01. <b>B)</b> Confocal microscopy of control and p110α knockdown cells shows reduced phagosome - lysosome fusion. Cells were loaded with Texas Red dextran, which was then chased into lysosomes. The graph to the right shows the numbers of phagosomes that were positive for a dextran signal. At least 100 phagosomes in each of three independent experiments were counted. Open bar = control cells, Solid bar = p110α knockdown cells. Means +/− s.e.m. are shown. *p<0.05.