Phagosomes isolated from p110α knockdown cells show normal recruitment and processing of the protease cathepsin D.

<p>Lysates from 4 and 6 hour phagosomes were prepared, separated by SDS-PAGE and transferred to nitrocellulose membrane. Blots were probed with an antibody for cathepsin D. Actin is shown as a loading control. Although a trend towards less cathepsin D in the phagosome lysates from p110α knockdown cells was observed, it was not found to be statistically significant. Empty bars = control cells, solid bars = p110α knockdown cells. Data are means +/− s.e.m., from four independent experiments.