Quantification of co-localization of BCG containing phagosomes with lysosomes in AZP treated THP-1 cells.

<p>(<b>A</b>) Co-localization of BCG containing phagosomes with lysosomes in AZP treated and untreated cells were quantified microscopically by acquisition of LAMP-1 marker of late endosome/lysosome by BCG phagosomes. THP-1 cells infected with <i>M. bovis</i> BCG-GFP (green) were exposed to AZP for 24 h and stained with antibody against LAMP-1 (red) followed by secondary antibody Alexa Fluor 594 and DAPI (blue) for nuclei staining. (<b>B</b>) A representative cell showing co-localization of BCG-containing phagosome with LAMP-1 marker. THP- cells infected with BCG-GFP only were used as control. Data shown are the average of 3 individual experiments. Significance was referred as* for <i>p</i><0.005.