Interaction of phagosomes containing different phagocytic particles with MVB/Late endosomes.

<p>LBPA acquisition was used to assess the interaction of phagosomes containing different particles with MVB/late endosomes. <b>A</b>) LBPA staining of a cell containing a LBPA-negative phagosome. <b>B</b>) Internalized agRBC stained with CFSE. <b>C</b>) Corresponding merged image. <b>D</b>) LBPA staining of a cell stably expressing the FcRγ-IIA and containing three LBPA-positive phagosomes. <b>E</b>) Corresponding differential interference contrast (DIC) image. <b>F</b>) Corresponding merged image. Arrows indicate three LBPA-positive phagosomes and arrowhead indicate a negative phagosome after 30 min pulse (0 min chase time). Bars, 10 µm. <b>G</b>) Quantification of the LBPA-positive phagosomes. Wild type and engineered SMCs were exposed to different phagocytic particles for 30 min and then chased for the times indicated in the graph abscissa. After fixation the cells were stained with LBPA antibodies and analyzed under a confocal microscope. Data shows the percentage of LBPA-positive phagosomes and are means ± SEM of, at least, three independent experiments. At each time point 100 phagosomes were analyzed. *, p < 0.05 and **, p < 0.01 comparing differences between LBPA-acquisition by phagosomes with agRBC and with IgG-opsonized latex beads.