Analysis of ERK1,2 and p38MAPK phosphorylation status.
- Yali Cao (312799)
- Jiajin Yang (312803)
- Kerry Colby (312808)
- Susan L. Hogan (312812)
- Yichun Hu (312814)
- Caroline E. Jennette (312815)
- Elisabeth A. Berg (312816)
- Youkang Zhang (312817)
- J. Charles Jennette (312820)
- Ronald J. Falk (312822)
- Gloria A. Preston (312827)
Publication date
February 2013
DOIAbstract
<p>Bar graphs represent the ratio of intensity pERK/ERK and pp38/p38MAPK as quantitated by densitometric scanning analysis using ImageMaster VDS software. Western blot analysis for ERK1,2 and p38MAPK activation demonstrates PTPN22 gain-of-function (R620) exerts a negative effect on the ERK signaling pathway, compared to loss-of-function (R263Q) and non-variant controls. In contrast p38 mitogen-activated protein kinase (p38 MAPK) was increased with the gain-of-function (R620) phenotype.</p
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