Analysis of extracellular signal-regulated kinase (ERK) -1 and -2 activities via Western blot.

<p>(A) Tissue lysates from lumbar mammary glands at day 21 and day 28 were probed with an antibody recognizing phosphorylated (activated) and total (phosphorylated and non-phosphorylated) ERK1 (also known as p44, upper band 44kDa) and ERK2 (also known as p42, lower band 42kDa). Amidoblack (Ambl) served as control. (B+C) Densitometric analysis presented as the ratio of activated ERK-1/-2 to total ERK at day 21 (B) and day 28 (C). LP = low protein (white bars); NP = normal protein (black bars); d = day; * = p<0.05, ** = p<0.01.