Effect of erlotinib on directional invasion and proliferation of pII cells.

<p>Panel A indicates the net directional invasive movement (A – B; as defined in Fig. 1) of pII cells through agarose towards a source of EGF (50 ng/ml) measured in the absence (untreated; UT) or presence of erlotinib added directly into the well containing cells as described in Methods. Data for drug treated conditions are expressed as a % of the untreated control (set as 100%). Each histobar is the mean ± SEM for 5 independent determinations. Asterisks denote significant difference from untreated control with p≤0.05 (*) and p<0.005 (**). Panel B shows the effect of erlotinib on cell proliferation. Approximately 10⁴ pII cells were seeded in microwell plates and allowed to grow over 4 days in the presence of various concentrations of erlotinib as indicated. Cells were harvested and growth was determined by the MTT assay. Histobars represent means ± SEM of at least 3 independent determinations. Asterisk denotes significant difference from untreated control, with p<0.001.