Effects of reagents against EMT-related pathways on cell growth in HCC4006ER cells.

<p>A, HCC4006ER cells were treated for 72 hours with increasing concentrations of indicated reagents ± erlotinib. HCC4006 cells were treated for 72 hours with increasing concentrations of erlotinib for 72 hours to plot a reference curve. Data generated by cell viability assay (CellTiter-Glo) are expressed as a percentage of the value for untreated cells. The error bars represent SEM of 3 independent experiments. Combination index (CI) at IC50 dose of each combination treatment was calculated by CompuSyn software. CI>1, CI = 1, and CI<1 indicate antagonistic, additive and synergistic effects, respectively. B, HCC4006ER cells were treated for 72 hours with increasing concentrations of erlotinib, following treatment of PD173074 (1 μM), LY364947 (1 μM), LBH589 (10 nM), salinomycin (100 nM), or IWP2 (1 μM) for 14 days. Data generated by cell viability assay (CellTiter-Glo) are expressed as a percentage of the value for untreated cells. The error bars represent SEM of 3 independent experiments.