Humoral immune response observed in IFNAR^(−/−) mice vaccinated with heterologous prime boost vaccination with pcDNA3 and rMVA expressing VP2/VP7/NS1.

<p>The presence of antibodies specific of VP2, VP7, and NS1 in serum of immunized (A) and non-immunized (B) IFNAR^(−/−) mice was analyzed by ELISA. Sera from mice immunized with pcDNA3-VP2,-VP7,-NS1 (day 0) and rMVA-VP2,-VP7,-NS1 (day 14) and non-immunized were collected at days 0, 14, and 28 before the challenge with BTV-4, and dilution 1∶50 was analyzed by ELISA as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0034735#s4" target="_blank">Materials and Methods</a>. Standard deviations are shown as error bars. (C) BTV-4 neutralizing antibody detection in VP2/VP7/NS1 immunized mice by VNT. Neutralization titers at day 28 in sera of animals immunized with pcDNA3-VP2,-VP7,-NS1 (day 0) and rMVA-VP2,-VP7,-NS1 (day 14) (▪) and non-immunized (•) are shown. Means are presented (⁃) and standard deviations are shown as error bars.