Selectant VLPs elicit high-titer peptide-specific antibody responses but variable AMA1-reactive IgG titers.

<p>Groups of mice were immunized twice with 10mer selectant VLPs or wild-type MS2 VLPs. Serum was taken two-weeks following the boost. (A) Vaccine-elicited peptide titers were assessed by ELISA using recombinant PP7 bacteriophage VLPs displaying the NWDPTQFPGK peptide (in a surface-exposed AB loop similar to that of MS2) as the target antigen. (B) Antibody responses against native AMA1. In 3/10 vaccinated mice we observed low-titer cross-reactivity; these are referred to as “responders”. The remaining mice were characterized as “non-responders”. Mean OD₄₀₅ values are shown for each group. Error bars represent standard error of the mean, SEM.