Humoral immune response observed in IFNAR^(−/−) mice vaccinated with DNA/rMVA or rMVA/rMVA expressing VP2/NS1.

<p>Neutralizing antibodies specific of AHSV-4 or AHSV-9 were analyzed in sera of immunized mice by VNT. Neutralization titers at day 15 post-booster treatment in sera of animals immunized with DNA/rMVA (A) or rMVA/rMVA (B) expressing VP2, NS1, or VP2/NS1 are shown. (▴) Non-Immunized, (♦) VP2 immunized, (▪) NS1 immunized, and (•) VP2/NS1 immunized. Means are presented as bars (⁃). Asterisks (*) indicate statistically significant differences (<i>P</i><0.05) between immunized and non-immunized mice, calculated by signed rank test.