Serum antibody responses in mice vaccinated intranasally with iEEEV vaccine candidates.

<p>Groups of BALB/c mice (n = 10) were vaccinated once (graphs on left) or twice (graphs on right) with one of three iEEEV vaccine candidates (formalin-inactivated, INA-inactivated, or gamma irradiated) at doses ranging from 5–0.1 µg by the IN route. Serum was collected 21 d after each vaccination. Neutralizing antibody responses were determined by PRNT and serum antibody levels were determined by ELISA. In all graphs, dark bars represent the mean group titer 21 d after the first vaccination (n = 10); light bars represent the mean group titer 21 d after the second vaccination (n = 10). Standard error bars represent 2 times the SE of the mean (SE = SDxsqrt(n)).