Similar percentage of CD3⁺CD4⁺-cells in BE and duodenal <i>ex vivo</i> cultures.

<p>(<b>A</b>) <b>Comparison of the percentage of CD3⁺CD4⁺-cells in collagenated BE biopsies and </b><b><i>ex vivo</i></b><b> expanded T-cells.</b> The percentage of CD3⁺CD4⁺-cells was determined in paired biopsies from 5 BE patients treated by either collagenase or used for <i>ex vivo</i> expansion. Each bar represents the mean value±SEM of the percentage of CD3⁺CD4⁺-cells in the CD3⁺population (light grey bar: BE T-cells expanded for 14 days <i>ex vivo</i>, light grey with stripes: T-cells from collagenated BE biopsies). (<b>B</b>) <b>Percentage of CD3⁺CD4⁺-cells in </b><b><i>ex vivo</i></b><b> cultures of BE and duodenum.</b> The percentage of CD3⁺CD4⁺-cells from <i>ex vivo</i> cultures of 17 BE segments and 15 duodenal biopsies from 17 BE patients (DUO BE) and 11 duodenal biopsies from 11 controls (DUO control) was determined by flowcytometry. Each bar represents the mean value±SEM of the percentage of CD3⁺CD4⁺-cells in the CD3⁺ population (black bar: DUO control, dark grey bar: DUO BE, light grey bar: BE) (<b>ns</b> not significant).