Analysis of frequencies of CD4⁺CD25^highFOXP3⁺ T_reg cells after chemoimmunotherapy.

<p>(<b>A</b>) Flow cytometric analysis of CD25 and FOXP3 on peripheral blood derived CD4⁺ T cells from a representative colorectal cancer patient before (left panel) and after chemoimmunotherapy (right panel). Numbers represent percentage of events within the gate. (<b>B</b>) Serial analysis of frequencies of CD4⁺CD25^highFOXP3⁺ T_reg cells in colorectal cancer patients before and after chemoimmunotherapy. (<b>C</b>) Frequency of CD4⁺CD25^highFOXP3⁺ T_reg cells in 15 colorectal cancer patients before (light grey box) and after (dark grey box) chemoimmunotherapy. Shown here are median, 25^th and 75^th percentile (box), 10^th and 90^th percentile (whiskers) and outliers (dots), (*, p<0.05, Student's <i>t</i> test). (<b>D</b>) Serial analysis of total numbers of CD4⁺CD25^highFOXP3⁺ T_reg cells in colorectal cancer patients before and after chemoimmunotherapy.