Schematic maps and restriction enzyme digests of pHS300 and pHS400 plasmid DNA isolated from rBCG.

<p>(A) Schematic map of plasmid pHS300. The deleted region, found in plasmid DNA isolated from vaccine stocks of rBCG, is indicated by a thick, dotted line. (B) Lanes 2 & 7 contain plasmid DNA prepared prior to transformation into BCG (positive controls). Lanes 3–6 and 8–11 contain pHS300 plasmid DNA isolated from 4 different rBCG[pHS300] transformants. Plasmid DNA in lanes 2–6 was digested with restriction enzyme <i>Bgl</i>II and plasmid DNA in lanes 7–11 was digested with restriction enzymes <i>EcoR</i>V & <i>Apa</i>I. (C) Schematic map of plasmid pHS400. (D) Lanes 2 & 7 contain pHS400 plasmid DNA prepared prior to transformation into BCG (positive controls). Lanes 3–6 and 8–11 contain plasmid DNA isolated from 4 different rBCG[pHS400] (rBCG-Gag) transformants. Plasmid DNA in lanes 2–6 was digested with restriction enzyme <i>Bgl</i>II and plasmid DNA in lanes 7–11 was digested with restriction enzymes <i>Xho</i>I & <i>Apa</i>I. Results were the same for rBCG and rBCGΔ<i>panCD</i>.