<p>(<b>A</b>) LB, left border; AcMNPV, <i>Autographa Californica</i> Nuclear Polyhedrosis Virus; Ph-Pro, AcMNPV polyhedrin promoter; L, linker peptide (GPGP); poly A<sup>+</sup>, polyadenylation signal; RB, right border. (<b>B</b>) M, DNA marker DL 2000 (TakaRa); lane 1, recombinant plasmid digested with <i>Bam</i>HI and <i>Xho</i> I; lane 2, recombinant plasmid digested with <i>Bam</i>HI; lane 3, recombinant plasmid digested with <i>Xho</i> I; lane 4, PCR amplification of recombinant plasmid.</p
<p>Schematic of a portion of the <i>B. hermsii</i> linear plasmid lp28-1 contained in the wild-type ...
<p>The horizontal arrows indicate the translation orientation of the genes. The conserved core and i...
<p>(A) A linear DNA fragment containing the <i>pyrF</i> expression cassette as well as the entire se...
<p>(A) Schematic map of plasmid pHS300. The deleted region, found in plasmid DNA isolated from vacci...
<p>(A) The amplification of the whole <i>Catsper1</i> ORF by recombinant PCR. The sequences marked w...
<p>Separate amplification of the 5′ and 3′ ends of the MC58 <i>lpxL1</i> gene, introduction of a nov...
<p>(A) Modified plasmids derived from pCGxAF9VP2HRT, which containing segment A of the virulent stra...
<p>(<b>A</b>). Schematic showing the process of RCME (Watson 2008): (i) starting with a base strain ...
<p>PCR products of entry plasmids (pE) containing basic parts such as promoters (PR), coding sequenc...
The polyhedrin gene region of the Autographa californica nuclear polyhedrosis virus (AcMNPV) morphol...
<p>Abbreviations used: CG-P, a hybrid promoter carrying the <i>GAL10</i> upstream activating sequenc...
<p>(A) Plasmid pCHRGFP1: pPROBE-NT containing the <i>chr</i> promoter and <i>chrB</i> gene upstream ...
<p>1. DNA marker; 2. phoA-MT digested with NcoI and BamHI endonucleases (Up: Phoa plasmid, Down: MT)...
<p><b>A.</b> The digested products of three separate restriction digests of pCMS3 and pCambia 2301. ...
<p>Lane 1: The blank plasmid pET28a(+). Lane 2: The recombinant plasmid pET28a(+)–SjPGM or pET28a(+)...
<p>Schematic of a portion of the <i>B. hermsii</i> linear plasmid lp28-1 contained in the wild-type ...
<p>The horizontal arrows indicate the translation orientation of the genes. The conserved core and i...
<p>(A) A linear DNA fragment containing the <i>pyrF</i> expression cassette as well as the entire se...
<p>(A) Schematic map of plasmid pHS300. The deleted region, found in plasmid DNA isolated from vacci...
<p>(A) The amplification of the whole <i>Catsper1</i> ORF by recombinant PCR. The sequences marked w...
<p>Separate amplification of the 5′ and 3′ ends of the MC58 <i>lpxL1</i> gene, introduction of a nov...
<p>(A) Modified plasmids derived from pCGxAF9VP2HRT, which containing segment A of the virulent stra...
<p>(<b>A</b>). Schematic showing the process of RCME (Watson 2008): (i) starting with a base strain ...
<p>PCR products of entry plasmids (pE) containing basic parts such as promoters (PR), coding sequenc...
The polyhedrin gene region of the Autographa californica nuclear polyhedrosis virus (AcMNPV) morphol...
<p>Abbreviations used: CG-P, a hybrid promoter carrying the <i>GAL10</i> upstream activating sequenc...
<p>(A) Plasmid pCHRGFP1: pPROBE-NT containing the <i>chr</i> promoter and <i>chrB</i> gene upstream ...
<p>1. DNA marker; 2. phoA-MT digested with NcoI and BamHI endonucleases (Up: Phoa plasmid, Down: MT)...
<p><b>A.</b> The digested products of three separate restriction digests of pCMS3 and pCambia 2301. ...
<p>Lane 1: The blank plasmid pET28a(+). Lane 2: The recombinant plasmid pET28a(+)–SjPGM or pET28a(+)...
<p>Schematic of a portion of the <i>B. hermsii</i> linear plasmid lp28-1 contained in the wild-type ...
<p>The horizontal arrows indicate the translation orientation of the genes. The conserved core and i...
<p>(A) A linear DNA fragment containing the <i>pyrF</i> expression cassette as well as the entire se...