<p>(A) Plasmid pCHRGFP1: pPROBE-NT containing the <i>chr</i> promoter and <i>chrB</i> gene upstream of <i>gfp</i>. (B) Plasmid pCHRGFP2: pCHRGFP1 with an additional gentamicin resistance gene cloned into <i>mob</i> gene. Arrows indicate the direction of transcription. The restriction enzymes indicated were those used in this work.</p
<p><i>pAgpdi</i>: the <i>gpd</i> promoter with the <i>gpd</i> first intron derived from <i>A. bispor...
<p>Plasmids for gene over-expression (pDNB101), bacterial two-hybrid system (pDNB102 and pDNB103), b...
<p>Abbreviations used: CG-P, a hybrid promoter carrying the <i>GAL10</i> upstream activating sequenc...
<p>(A) One plasmid system. (B) Two plasmid system. In these designs, when Cre recombinase is express...
<p>(A) Full-length <i>gfp</i>-<i>AtRACK1C</i> in the pCB302 plasmid (pCB302-<i>gfp</i>-<i>AtRACK1C</...
<p>The red bars represent the homologous regions between different DNA fragments and vectors. The ye...
<p>(A) Six promoters, two viral (CMV and SV40) and four endogenous (SmHsp70, SmActin1, Sm23, and SmC...
<p>The boxes show the 5’ and 3’-end enzymes used for ligation of promoter candidates (PC). The left ...
<p>Schematic of a portion of the <i>B. hermsii</i> linear plasmid lp28-1 contained in the wild-type ...
<p>(A) Modified plasmids derived from pCGxAF9VP2HRT, which containing segment A of the virulent stra...
<p>(A) Genetic map of the IncQ-group conjugation plasmid pDSK5191. Protein coding sequences are show...
<p>(A) Schematic map of plasmid pHS300. The deleted region, found in plasmid DNA isolated from vacci...
<p>A) pSPLIT<i>rep</i><sup>1-219Rb-</sup>35S containing “modules” that could be removed and replaced...
<p>Separate amplification of the 5′ and 3′ ends of the MC58 <i>lpxL1</i> gene, introduction of a nov...
<p>Restriction enzymes used in generating the targeting plasmids are Kpn I (K), Hind III (H), BamHI ...
<p><i>pAgpdi</i>: the <i>gpd</i> promoter with the <i>gpd</i> first intron derived from <i>A. bispor...
<p>Plasmids for gene over-expression (pDNB101), bacterial two-hybrid system (pDNB102 and pDNB103), b...
<p>Abbreviations used: CG-P, a hybrid promoter carrying the <i>GAL10</i> upstream activating sequenc...
<p>(A) One plasmid system. (B) Two plasmid system. In these designs, when Cre recombinase is express...
<p>(A) Full-length <i>gfp</i>-<i>AtRACK1C</i> in the pCB302 plasmid (pCB302-<i>gfp</i>-<i>AtRACK1C</...
<p>The red bars represent the homologous regions between different DNA fragments and vectors. The ye...
<p>(A) Six promoters, two viral (CMV and SV40) and four endogenous (SmHsp70, SmActin1, Sm23, and SmC...
<p>The boxes show the 5’ and 3’-end enzymes used for ligation of promoter candidates (PC). The left ...
<p>Schematic of a portion of the <i>B. hermsii</i> linear plasmid lp28-1 contained in the wild-type ...
<p>(A) Modified plasmids derived from pCGxAF9VP2HRT, which containing segment A of the virulent stra...
<p>(A) Genetic map of the IncQ-group conjugation plasmid pDSK5191. Protein coding sequences are show...
<p>(A) Schematic map of plasmid pHS300. The deleted region, found in plasmid DNA isolated from vacci...
<p>A) pSPLIT<i>rep</i><sup>1-219Rb-</sup>35S containing “modules” that could be removed and replaced...
<p>Separate amplification of the 5′ and 3′ ends of the MC58 <i>lpxL1</i> gene, introduction of a nov...
<p>Restriction enzymes used in generating the targeting plasmids are Kpn I (K), Hind III (H), BamHI ...
<p><i>pAgpdi</i>: the <i>gpd</i> promoter with the <i>gpd</i> first intron derived from <i>A. bispor...
<p>Plasmids for gene over-expression (pDNB101), bacterial two-hybrid system (pDNB102 and pDNB103), b...
<p>Abbreviations used: CG-P, a hybrid promoter carrying the <i>GAL10</i> upstream activating sequenc...