Restriction enzyme mappings of the plasmids in the <i>C. tepidum</i> and <i>C. limnaeum</i> transconjugants.

<p>(A, B) Physical maps of the plasmids from the <i>C. tepidum</i> transconjugants. The maps were constructed with restriction enzymes <i>Eco</i>RI (A) and <i>Bgl</i>II (B). The restriction fragments were separated by agarose gel (1%) electrophoresis. The control plasmids pDSK5191 (lane 1), pDSK5191-<i>cycA</i> (lane 2), and pDSK5191-<i>soxB</i> (lane 3) were obtained from the donor S17-1 cultures. Lanes 4-11 are the plasmid samples of the <i>C. tepidum</i> cultures. The genotype of <i>C. tepidum</i> is indicated above each lane. The bars and numbers at the left side of the panel indicate mobility and size of the <i>Sty</i>I digests of the λ-phage DNA. (C) Physical maps of the plasmids from <i>C. limnaeum</i> transconjugants. The maps were constructed with restriction enzymes <i>Eco</i>RI (lanes 1–3) and <i>Bam</i>HI (lanes 4–6). Lanes 1 and 4 are pDSK5192 plasmids prepared from the donor S17-1 cultures. Lanes 2-3 and 5-6 are the plasmid samples of the <i>C. limnaeum</i> cultures. The genotype of <i>C. limnaeum</i> is indicated above each lane. The bars and numbers at the left side of the panel indicate mobility and sizes of the <i>Sty</i>I digests of the λ-phage DNA, respectively.