Rapamycin reduces tubulointerstitial injuries and collagen deposition in obstructed kidneys.

<p>Animals were treated as decribed above. A(A′)–H(H′): Representative sections of vehicle (A–D:low power, inserted A′–D′: high power) or rapamycin-treated (E-H: low power, inserted E′–H′: high power) kidney with hematoxylin-eosin staining. Scale Bar: 200 µm. I–P: Representative costaining sections of obstructed kidneys with vehicle (M–P) or rapamycin (I–L) treatment, using anti-collagen I (red) and anti-LTL (green) as primary antibodies. Scale bar: 50 µm. Q–S: Quantitative assessment of tubular injuries (Q), interstitial infiltrates (R) and collogen-I deposition (S) in kidneys derived from UUO mice with or without administration of rapamycin. **P<0.01 vs. vehicle treated groups, NS no significance. n = 5 animals for each group. Error bars represent S.E.