Assessment of myofibroblasts activation in obstructed nephropathy.

<p>Animals were treated as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0033626#s2" target="_blank">Methods</a> and Materials. A. Representative sections of vehicle or rapamycin-treated kidneys stained with anti-αSMA by immunohistochemistry. Scale bar: 100 µm. B. Representative confocal images of vehicle or rapamycin-treated kidneys costained with anti-vimentin (red) and anti-LTL(green). Scale bar: 100 µm. C–F: Representative western blot and densitometric analyses for αSMA (C–D) and vimentin (E–F) expression in UUO kidneys. β-actin was used in this experiment to control for equal protein loading. Data were presented as mean ± S.E.M. n = 5 animals in each group. *P<0.05, **P<0.01 vs. vehicle-treated groups. NS no significance. Error bars represent S.E.