No diet-induced difference was found in the proportion of CD4⁺Foxp3⁺ T cells.

<p>(<b>A</b>) Representative plot of lymphocyte and CD3⁺CD4⁺ gate. (<b>B</b>) Bars represent the proportion of Foxp3⁺ cells (gated on 100% CD4⁺CD3⁺ cells) in various lymphoid compartments. No diet-induced differences was found in the proportion of CD4⁺Foxp3⁺ T cells in all lymphoid compartments tested. Data are presented as mean values ± SEM of two independent experiments with 6 mice in each group. (<b>C</b>) Representative histogram from PLN, showing the proportion of CD4⁺Foxp3⁺T cells in the STD diet mice compared to the GF diet mice. Spleen (S); inguinal lymph nodes (ILN); mesenteric lymph nodes (MLN); Peyer's patches (PP) and pancreatic lymph nodes (PLN). Black bars: STD diet. Grey bars: GF diet.