The proportion of CD4⁺T cells expressing CD45RB^high and CD45RB^low was influenced by the diet.

<p>(<b>A</b>) Bars represent percentages of CD45RB^low expressing cells gated on 100% CD3⁺CD4⁺ cells. There was a decreased proportion of CD4⁺ CD45RB^low T cells in S and PP of BALB/c mice receiving the STD diet compared to the GF diet. (<b>B</b>) Percentages of cells expressing CD45RB^high gated on 100% CD3⁺CD4⁺ cells. There was an increased proportion of CD4+CD45RB^high T cells in PP and PLN in mice receiving the STD diet. Data are presented as mean values ± SEM of two independent experiments with 6 mice in each group. (<b>C</b>) Representative dot plot of cells isolated from PLN showing the proportion of CD45RB^low and CD45RB^high CD4⁺ T cells from STD mice vs. GF mice. Spleen (S); inguinal lymph nodes (ILN); mesenteric lymph nodes (MLN); Peyer's patches (PP) and pancreatic lymph nodes (PLN). Black bars: STD diet. Grey bars: GF diet. * P<0.05. ** P<0.01.