Effects of dietary restriction on T cell development phenotype in thymus A) FACS plot of CD4 against CD8 in the first panel and the corresponding CD4⁻CD8⁻ cellularity graph.

<p>The second panel represents the CD3/FSC FACS profiles of CD4⁻CD8⁻ DN fractions along with the corresponding cellularity graph of DN CD3⁻. The third panel of plots shows CD44/CD25 profiles of DN CD3⁻ fraction. This panel shows the DN1-DN4 stages in the dietary intervention groups. B) FACS plots show the expression of CD3/CD69 profiles of CD8⁺CD4⁻ fractions representing the CD3⁻CD69⁻ISP and CD3⁺CD8⁺ SP cells C) The upper and lower panel of graphs show the phenotypic changes taking place during the development stages starting from DN stages to CD3⁻CD69⁻ ISP, CD4⁺CD8⁺ DP and CD4⁺ SP and CD8⁺ SP. Fig. 4A and C *, # = p<0.05, ** = p<0.001, *** = p<0.007. n = 8/group. Ad libitum, 2 weeks 30% DR and 3 days fasting groups are represented by black, white and grey box, respectively.